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Synlett
DOI: 10.1055/a-2685-9396
Letter

Pseudouridine Modified Phosphorodiamidate Morpholino Oligonucleotides: Synthesis and Biophysical Insights

Subhamoy Pratihar
1   School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Kolkata, India (Ringgold ID: RIN62397)
,
Debajyoti Kuilya
1   School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Kolkata, India (Ringgold ID: RIN62397)
,
Md Qasim
1   School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Kolkata, India (Ringgold ID: RIN62397)
,
Arnab Das
1   School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Kolkata, India (Ringgold ID: RIN62397)
,
Surajit Sinha
1   School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Kolkata, India (Ringgold ID: RIN62397)
› Author Affiliations
Supported by: Department of Science and Technology New Delhi India DST/TDT/TC/RARE/2022/10c2
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The synthesis of Morpholino Pseudouridine chlorophosphoramidate monomer from Pseudouridine and its incorporation in a 12-mer PMO (Phosphorodiamidate Morpholino Oligonucleotide) has been successfully achieved. Thermal melting (Tm) of this PMO was performed with the complementary strand of DNA and RNA. In the case of RNA, the Tm of base pairing between Pseudouridine with A and G was comparable. Circular Dichroism analysis reveals the B-type helical conformation of PMO-RNA duplexes. These advancements are expected to broaden the potential applications of PMOs in biomedical research.



Publication History

Received: 31 July 2025

Accepted after revision: 18 August 2025

Accepted Manuscript online:
19 August 2025

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